Publications

Stevens, T. A., Tomaleri, G.P., Hazu, M, Wei, S., Nguyen, V. N., DeKalb, C., Voorhees, R.M., Pleiner, T. A nanobody-based strategy for rapid and scalable purification of native human protein complexes bioRxiv doi:10.1101/2023.03.09.531980
Guna, A., Page, K., Replogle, J. R., Esantsi, T. K., Wang, M. L., Weissman, J. S., Voorhees, R. M., A dual sgRNA library design to probe genetic modifiers using genome-wide CRISPRi screens bioRxiv doi: 10.1101/2023.01.22.525086
Pleiner, T., Hazu, M., Tomaleri, G.P., Nguyen, V. N., Januszyk K., Voorhees, R.M. (2022) "A selectivity filter in the EMC limits protein mislocalization to the ER" bioRxiv doi: 10.1101/2022.11.29.518402
Inglis, A.J., Guna, A., Merchán, A.G., Pal, A. Esantsi, T.K., Keys, H.R., Frenkel, E.M., Oania, R.S., Weissman, J.S. Voorhees, R.M. (2022) "Coupled protein quality control during nonsense mediated mRNA decay." bioRxiv doi: 10.1101/2021.12.22.473893

Guna, A., Stevens T., Inglis, A., Replogle, J., Esantsi, T., Muthukumar, G., Shaffer, K., Wang, M., Pogson, A., Jones, J., Lomenick, B., Chou, T-F., Weissman, J., Voorhees, R..M. (2022) "MTCH2 is a mitochondrial outer membrane protein insertase" Science, 378(6617) doi: 10.1126/science.add1856 bioRxiv doi: 10.1101/2022.09.15.508165 pmid: 36264797
Guna, A., Hazu M., Tomaleri, G.P., Voorhees, R.M. (2022) "A TAle of Two Pathways: Tail-Anchored Protein Insertion at the Endoplasmic Reticulum" Cold Spring Harbor Perspectives in Biology doi: 10.1101/cshperspect.a041252 pmid: 36041783
McCord, R.P., Taipale, M., Teichmann, S., Voorhees, R.M., Wan, Y., Dekker, C., Sanjana, N.E. (2022) Voices on technology: The molecular biologists' ever-expanding toy box Mol Cell, 82(2): P221-226. doi: 10.1016/j.molcel.2021.12.025 pmid: 35063087

Pleiner, T., Hazu, M., Tomaleri, G.P., Januszyk, K., Oania, R.S., Sweredoski, M.J., Moradian, A., Guna, A., Voorhees, R.M. (2021) "WNK1 is an assembly factor for the human ER membrane protein complex." Mol Cell, 81(13): 2693-2704.e12. pmid: 33964204. pmcid: PMC8254792. bioRxiv doi: 10.1101/2020.07.20.213066
Stangherlin, A., Wong, D.C.S., Barbiero, S., Watson, J.L., Zeng, A., Seinkmane, E., Chew, S.P., Beale, A.D., Hayter, E.A., Guna, A., Inglis, A.J., Bartolami, E.M.S., Lequeux, N., Pons, T., Day, J., van Ooijen, G., Voorhees, R.M., Bechtold, D.A., Derivery, E., Edgar, R.S., Newham, P., O'Neill, J.S. (2021) "Compensatory ion transport buffers daily protein rhythms to regulate osmotic balance and cellular physiology" Nature Communications, In Press. doi: 10.1101/2020.05.28.118398. pmid 34654800

Inglis, A.J., Page, K.R., Guna, A., Voorhees, R.M. (2020) "Differential Modes of Orphan Subunit Recognition for the WRB/CAML Complex." Cell Rep, 30(11): 3691-3698.e5. doi: 10.1016/j.celrep.2020.02.084. pmid: 32187542. pmcid: PMC7147533. doi bioRxiv: 10.1101/828228
Pleiner, T.*, Pinton Tomaleri, G.*, Januszyk, K.*, Inglis, A.J., Hazu, M., Voorhees, R.M. (2020) "Structural basis for membrane insertion by the human ER membrane protein complex" Science, 369(6502): 433-436. doi: 10.1126/science.abb5008. pmid: 32439656. pmcid: PMC7547852. Full text. Downloads:
- Protocol for native purification of GFP-tagged proteins and protein complexes
Banerjee, A.K., Blanco, M.R., Bruce, E.A., Honson, D.D., Chen, L.M., Chow, A., Bhat, P., Ollikainen, N., Quinodoz, S.A., Loney, C., Thai, J., Miller, Z.D., Lin, A.E., Schmidt, M.M., Stewart, D.G., Goldfarb, D., De Lorenzo, G., Rihn, S.J., Voorhees, R.M., Botten, J.W., Majumdar, D., Guttman, M. (2020) "SARS-CoV-2 Disrupts Splicing, Translation, and Protein Trafficking to Suppress Host Defenses." Cell, in press. doi: 10.1016/j.cell.2020.10.004. pmid: 33080218. pmcid: PMC7543886.

Voorhees, R.M., Hegde, R.S. (2016) "Structure of the Sec61 channel opened by a signal sequence." Science, 351(6268): 88-91. doi: 10.1126/science.aad4992. pmid: 26721998. pmcid: PMC4700591.
Voorhees, R.M., Hegde, R.S. (2016) "Toward a structural understanding of co-translational protein translocation." Curr. Opin. Cell Biol., 41: 91-9. doi: 10.1016/j.ceb.2016.04.009. pmid: 27155805.

Voorhees, R.M., Hegde, R.S. (2015) "Structures of the scanning and engaged states of the mammalian SRP-ribosome complex." eLife, 4. doi: 10.7554/eLife.07975. pmid: 26158507. pmcid: PMC4497383.

Voorhees, R.M.†*, Fernández, I.S.*, Scheres, S.H., Hegde, R.S. (2014) "Structure of the mammalian ribosome-Sec61 complex to 3.4 Å resolution." Cell, 157(7): 1632-43. doi: 10.1016/j.cell.2014.05.024. pmid: 24930395. pmcid: PMC4081569. †: corresponding author.

Voorhees, R.M., Mandal, D., Neubauer, C., Köhrer, C., RajBhandary, U.L., Ramakrishnan, V. (2013) "The structural basis for specific decoding of AUA by isoleucine tRNA on the ribosome." Nat. Struct. Mol. Biol., 20(5): 641-3. doi: 10.1038/nsmb.2545. pmid: 23542153. pmcid: PMC3672977.
Voorhees, R.M., Ramakrishnan, V. (2013) "Structural basis of the translational elongation cycle." Annu. Rev. Biochem., 82: 203-36. doi: 10.1146/annurev-biochem-113009-092313. pmid: 23746255.

Schmeing, T.M.*, Voorhees, R.M.*, Kelley, A.C., Ramakrishnan, V. (2011) "How mutations in tRNA distant from the anticodon affect the fidelity of decoding." Nat. Struct. Mol. Biol., 18(4): 432-6. doi: 10.1038/nsmb.2003. pmid: 21378964. pmcid: PMC3072312.

Voorhees, R.M.*, Schmeing, T.M.*, Kelley, A.C., Ramakrishnan, V. (2010) "The mechanism for activation of GTP hydrolysis on the ribosome." Science, 330(6005): 835-8. doi: 10.1126/science.1194460. pmid: 21051640. pmcid: PMC3763471.

Voorhees, R.M., Weixlbaumer, A., Loakes, D., Kelley, A.C., Ramakrishnan, V. (2009) "Insights into substrate stabilization from snapshots of the peptidyl transferase center of the intact 70S ribosome." Nat. Struct. Mol. Biol., 16(5): 528-33. doi: 10.1038/nsmb.1577. pmid: 19363482. pmcid: PMC2679717.
Schmeing, T.M.*, Voorhees, R.M.*, Kelley, A.C., Gao, Y.G., Murphy, F.V., Weir, J.R., Ramakrishnan, V. (2009) "The crystal structure of the ribosome bound to EF-Tu and aminoacyl-tRNA." Science, 326(5953): 688-94. doi: 10.1126/science.1179700. pmid: 19833920. pmcid: PMC3763470.

Kingery, D.A.*, Pfund, E.*, Voorhees, R.M.*, Okuda, K., Wohlgemuth, I., Kitchen, D.E., Rodnina, M.V., Strobel, S.A. (2008) "An uncharged amine in the transition state of the ribosomal peptidyl transfer reaction." Chem. Biol., 15(5): 493-500. doi: 10.1016/j.chembiol.2008.04.005. pmid: 18482701. pmcid: PMC2851197.
Weixlbaumer, A.*, Jin, H.*, Neubauer, C., Voorhees, R.M., Petry, S., Kelley, A.C., Ramakrishnan, V. (2008) "Insights into translational termination from the structure of RF2 bound to the ribosome." Science, 322(5903): 953-6. doi: 10.1126/science.1164840. pmid: 18988853. pmcid: PMC2642913.